Tetracycline-inducible Expression Systems New The goal of this thesis was to create a mouse model permitting the analysis of neuregulin-1 (NRG1) function in the adult nervous system. Key words tetracycline-inducible expression system; Cre/lox P system; transgenic mouse modeling; leaky expression; stringent control; lineage-specific gene expression; lineage-specific RNAi.
Inducible Cell-targeted Mutations Tetracycline Gene Regulation. NRG1 is a polypeptide growth factor, which contains an ‘epidermal growth factor like’ (EGFL) domain and serves as a land for the erb B receptor tyrosine kinase family. Inducible, Cell - targeted Mutations in Mice. We use the tetracycline gene regulation system, which is a two-gene system. One gene expresses the tetracycline.
TET Controlled Gene Expression Genetiy Modified Models. Tetracycline (TET) technology allows precise, reversible, and efficient spatiotemporal control of gene expression. Bujard and colleagues at the University of Heidelberg, and is owned by TET Systems Holding Gmb H & Co. The TET technology was first described for use in 1995 (M. Science) and consequently utilized many times to analyze the results of gene expression after the onset of a disease. Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Gene expression is induced in the presence of tetracycline. Tetracycline technology allows precise, reversible, and efficient. TET-regulated mouse and rat models have a very strong track record of success more than.
Characterization of Tetracycline-Inducible Bitransgenic Krt12rtTA/+/. These mice enable regulated expression of mutant APP using a tet-off system. Ribosomal entry site–reverse tetracycline transcription activa- tor IRES-rtTA cassette was. Krt12 promoter has failed to produce a transgenic mouse line.
Rating: 95 / 100
Overall: 94 Rates